P-169: Protective Effect of Vitamin E on Cypermethrin - Induced Damages Correlates with P53 Gene Expression and Nitrosative Stress

Background: Because of fast rate of degradation and low mammalian cells toxicity the cypermethrin (CPM) is a wide used insecticide in domestic agriculture and in veterinary medicine applications. The compound exerts its pathological impact by down-regulating the antioxidant status. Therefore, current study was designed to evaluate the protective effect of vitamin E on CPMinduced apoptosis and nitrosative stress on ovarian tissue. Materials and Methods: Eighteen Mature female rats were divided into three (N=6) test and control-sham groups. The animals in test group 1 received CPM (75 mg/kg) and in test group 2 received vitamin E (150 mg/ kg) + CPM by gavages, for 24 constitutive days. The normal saline was administrated in control-sham group. After evaluating the total RNA content the P53 gene expression was evaluated by RT-PCR. The tissue total protein (TP), total antioxidant capacity (TAC) and nitric oxide (NO) were examined. Results: Vitamin E administrated animals were manifested with significantly (p<0.05) higher total RNA (3986.33 ± 24.79) and protein (3.25 ± 0.03) contents versus the CPM alone-administrated (1847.34 ± 19.94 for RNA and 2.79 ± 0.12 for protein) group. The P53 gene expression was detected in CPM alone group while it was not observed in vitamin E-administrated group. The vitamin E up-regulated TP and TAC and reduced NO level compared to CPM alone group. Conclusion: Our data suggest that CPM impact the ovarian tissue by inducing the nitrosative stress which promotes the P53 gene which in turn leads to cellular apoptosis. On the other hand vitamin E by up-regulating antioxidant status inhibited the damages.